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Pré-Publication, Document De Travail Année : 2022

Calibrated photoacoustic spectrometer based on a conventional imaging system for in vitro characterization of contrast agents

Résumé

Photoacoustic (PA) imaging systems are spreading in the biomedical community and the development of new PA contrast agents is an active area of research. However, PA contrast agents are usually characterized with spectrophotometry or uncali-brated PA imaging systems, leading to partial assessment of their PA efficiency. To perform quantitative PA spectroscopy of contrast agents in vitro, we have developed a calibration method adapted to conventional PA imaging systems. Contrast agents in solution are injected in a dedicated non-scattering tube phantom imaged at different optical wavelengths. The calibration method uses a reference solution of cupric sulfate to simultaneously correct for the spectral energy distribution of excitation light at the tube location and perform a conversion of the tube amplitude in the image from arbitrary to spectroscopic units. It was implemented on a conventional imaging set-up based on a tunable laser operating between 680 nm and 980 nm and a 5 MHz clinical ultrasound array. We demonstrated robust calibrated PA spectroscopy with 15 μL sample volumes of known chromo-phores and commonly used contrast agents. The validated method will be an essential tool for the development of new and efficient PA contrast agents, by improving their quantitative characterization.
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Dates et versions

hal-02509232 , version 1 (16-03-2020)
hal-02509232 , version 2 (27-04-2021)
hal-02509232 , version 3 (08-07-2022)
hal-02509232 , version 4 (01-09-2022)

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Théotim Lucas, Mitradeep Sarkar, Yoann Atlas, Clément Linger, Gilles Renault, et al.. Calibrated photoacoustic spectrometer based on a conventional imaging system for in vitro characterization of contrast agents. 2022. ⟨hal-02509232v3⟩
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