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Pré-Publication, Document De Travail Année : 2021

Calibrated photoacoustic spectrometer for in vitro characterization of contrast agents based on a standard imaging system

Résumé

Photoacoustic (PA) contrast agents are usually characterized with spectrophotometry or uncalibrated PA imaging systems, leading to partial assessment of their PA efficiency. To perform calibrated PA spectroscopy with a PA imaging system, we developed a method that simultaneously corrects for the spectral energy distribution of excitation light and performs a conversion from arbitrary to spectroscopic units, using a calibration solution of cupric sulfate. The method was implemented on a standard imaging set-up based on a tunable laser operating between 680nm and 980nm and a 5MHz clinical ultrasound array. We demonstrated robust calibrated PA spectroscopy with 15μL sample volumes of known chromophores and commonly used contrast agents, and for up to 4 samples simultaneously. The detection sensitivity was evaluated to be below 0.05 cm -1 in the range 680-930 nm.
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Dates et versions

hal-02509232 , version 1 (16-03-2020)
hal-02509232 , version 2 (27-04-2021)
hal-02509232 , version 3 (08-07-2022)
hal-02509232 , version 4 (01-09-2022)

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Mitradeep Sarkar, Théotim Lucas, Yoann Atlas, Gilles Renault, Florence Gazeau, et al.. Calibrated photoacoustic spectrometer for in vitro characterization of contrast agents based on a standard imaging system. 2021. ⟨hal-02509232v2⟩
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