Due to its capacity to store or supply energy with high power, the supercapacitor is becoming an attractive component. Because of the electrostatic nature of energy storage, the endurance of this component toward repetitive charge and discharge cycles is relatively high. The goal of this paper is to demonstrate that the cycling has an impact on the degradation of the supercapacitor and as a result on its lifetime. Based on accelerated cycling tests, some supercapacitors were studied using a dedicated test bench. Temperature, voltage, and current, that are the parameters which accelerate ageing, are monitored. In fact, observations during cycling tests show an important acceleration of the degradation compared to a similar static test having the same voltage and core temperature but without cycling. This paper proposes a method to quantify the acceleration of ageing during cycling phase.