Exploring the fluxotype of the E. coli Y-ome using high-resolution fluxomics - Archive ouverte HAL
Communication Dans Un Congrès Année : 2023

Exploring the fluxotype of the E. coli Y-ome using high-resolution fluxomics

Résumé

We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software tools. This workflow allowed us to obtain highly detailed maps of carbon fluxes in the central carbon metabolism in a fully automated manner. It was applied to investigate the glucose fluxotypes of 180 Escherichia coli strains deleted for y-genes. Since the products of these y-genes potentially play a role in a variety of metabolic processes, the experiments were designed to be agnostic as to their potential metabolic impact. The obtained data highlight the robustness of E. coli’s central metabolism to y-gene deletion. For two y-genes, deletion resulted in significant changes in carbon and energy fluxes, demonstrating the involvement of the corresponding y-gene products in metabolic function or regulation. This work also introduces novel metrics to measure the actual scope and quality of high-throughput fluxomics investigations.
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Dates et versions

hal-04282012 , version 1 (13-11-2023)

Identifiants

  • HAL Id : hal-04282012 , version 1

Citer

Cécilia Bergès, Edern Cahoreau, Pierre Millard, Brice Enjalbert, Mickael Dinclaux, et al.. Exploring the fluxotype of the E. coli Y-ome using high-resolution fluxomics. Séminaire TBI Toulouse, Mar 2023, Toulouse (31000), France. ⟨hal-04282012⟩
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