We consider fibrations by affine lines on smooth affine surfaces obtained as complements of smooth rational curves $B$ in smooth projective surfaces $X$ defined over an algebraically closed field of characteristic zero. We observe that except for two exceptions, these surfaces $X \setminus B$ admit infinitely many families of $\mathbb{A}^1$-fibrations over the projective line with irreducible fibers and a unique singular fiber of arbitrarily large multiplicity. For $\mathbb{A}^1$-fibrations over the affine line, we give a new and essentially self-contained proof that the set of equivalence classes of such fibrations up to composition by automorphisms at the source and target is finite if and only if the self-intersection number of $B$ in $X$ is less than or equal to 6.