Article Dans Une Revue International Journal of Biological Macromolecules Année : 2026

Exploring the multifunctional potential of tannic acid-crosslinked Wharton's jelly hydrogel as a scaffold for bone regenerative medicine

Vincent Ball
F. Boulmedais

Résumé

Rather than directly stimulating osteoblastic differentiation, scaffolds with antioxidant and immunomodulatory properties emerge as a promising strategy in bone regenerative medicine. Wharton's jelly (WJ) matrix exhibits intrinsic immunomodulatory potential, but its clinical use is limited by poor mechanical stability and susceptibility to reactive oxygen species (ROS). To address these limitations, tannic acid (TA) is used as a bioactive cross-linker. Two approaches are explored: TA incorporation and in situ oxidation. TA diffuses through WJ hydrogel (9.04 ± 2.91 mm2/h), while in situ oxidation at the hydrogel edge markedly increases the elastic modulus (∼100-folds) and enhances enzymatic stability. Oxidized (o)-TA-WJ scaffolds act as biocompatible carriers, releasing TA, exhibiting antioxidant activity, reducing intracellular ROS accumulation in H2O2-stimulated fibroblasts, and inhibiting S. aureus and P. aeruginosa growth and adhesion. In addition, these scaffolds modulate macrophage polarization towards a hybrid M1/M2 phenotype. In vivo, o-TA-WJ scaffolds demonstrate high tissue compatibility and promote bone regeneration in a critical-sized rat calvarial defect model. Overall, TA-cross-linked WJ scaffolds combine mechanical reinforcement with antioxidant, antibacterial and osseoregenerative functions, highlighting their strong potential as multifunctional platform for bone tissue engineering.

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Dates et versions

hal-05571273 , version 1 (28-03-2026)

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Citer

Loïc Scomazzon, Lorinne Adam, Anaïs Lavrand, Flora Lemaire, Fabien Lamret, et al.. Exploring the multifunctional potential of tannic acid-crosslinked Wharton's jelly hydrogel as a scaffold for bone regenerative medicine. International Journal of Biological Macromolecules, 2026, 346, pp.150500. ⟨10.1016/j.ijbiomac.2026.150500⟩. ⟨hal-05571273⟩
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