DNA extraction for unsorted bulk river samples v1
Résumé
The objective of this protocol is to propose an unsorted bulk DNA extraction methods for samples collected in aquatic environment such as kick-net samples. These community DNA extractions can then be used to describe various aspect of these communities using for example metabarcoding approaches. The protocol starts with a sample preserved in ethanol. The different steps of the protocol are: replacing the ethanol by a TNES Buffer, grinding the sample, DNA extraction with the Qiagen PowerSoil Pro kit. This protocol has been optimized and tested on mock samples composed of macrozobenthos organisms, algae, bryophytes, leaf litter, sand, gravels, and ethanol.