Article Dans Une Revue Veterinary Research Année : 2025

Curcumin prevents dexamethasone-induced activation of the pseudorabies virus in rat pheochromocytoma cells through the miR-155-5p-Aak1-Numb/Notch2 signalling axis

Naixiu Wang
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Fan Yang
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Zhiyun Qiu
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Lin Zhang
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Dingqiu Zou
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Yanru Tang
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Ruihan Zhang
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Chenlu Sun
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Pei Liu
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Kexin Qi
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Jingyi Wang
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Hua He
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Ling Gan
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Résumé

AbstractPseudorabies virus (PRV) causes neurological disorders and organ damage in diseased animals. After initial infection, PRV activity is gradually inhibited; however, stress stimulation increases the host’s glucocorticoid levels, which overcomes the inhibition of PRV activity. Curcumin (Cur) helps maintain the inhibitory state of the Epstein-Barr virus, although further research is needed to establish whether Cur can prevent PRV activation triggered by stress hormones. In this study, we used PC-12 cells to determine the effects of Cur on PRV activation. The cells were successfully infected with PRV at a multiplicity of infection of 1 for 24 h, resulting in the inhibition of PRV activity. Following incubation with 0.5 µM dexamethasone (DEX) for 4 h, the inhibition of PRV activity was blocked. Further mechanistic analyses using a dual-luciferase assay revealed that miR-155-5p directly targets and regulates Aak1 and its downstream signalling molecules, Numb and Notch2, in maintaining and disrupting PRV inhibition. Moreover, in vitro experiments using miR-155-5p mimics and inhibitors, combined with Aak1 overexpression and interference, confirmed that the miR-155-5p-Aak1-Numb/Notch2 axis prevented DEX-induced disruption of PRV inhibition by Cur. These findings provide a novel regulatory target for preventing stress-activated PRV and provide evidence for the potential use of Cur as a stress modulator in practical applications.

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hal-05048495 , version 1 (28-04-2025)

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Naixiu Wang, Fan Yang, Zhiyun Qiu, Lin Zhang, Dingqiu Zou, et al.. Curcumin prevents dexamethasone-induced activation of the pseudorabies virus in rat pheochromocytoma cells through the miR-155-5p-Aak1-Numb/Notch2 signalling axis. Veterinary Research, 2025, 56 (1), pp.86. ⟨10.1186/s13567-025-01509-9⟩. ⟨hal-05048495⟩

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