Identifiability of heterogeneous phenotype adaptation from low-cell-count experiments and a stochastic model - Archive ouverte HAL
Pré-Publication, Document De Travail Année : 2024

Identifiability of heterogeneous phenotype adaptation from low-cell-count experiments and a stochastic model

Résumé

Adaptive resistance contributes significantly to treatment failure in many cancers. Despite the increased prevalence of experimental studies that interrogate this phenomenon, there remains a lack of applicable quantitative tools to characterise data, and importantly to distinguish between resistance as a discrete phenotype and a (potentially heterogeneous) continuous distribution of phenotypes. To address this, we develop a stochastic individual based model of adaptive resistance in low-cell-count proliferation assays. That our model corresponds probabilistically to common partial differential equation models of resistance allows us to formulate a likelihood that captures the intrinsic noise ubiquitous to such experiments. We apply our framework to assess the identifiability of key model parameters in several population-level data collection regimes; in particular, parameters relating to the adaptation velocity and within-population heterogeneity. Significantly, we find that heterogeneity is practically non-identifiable from both cell count and proliferation marker data, implying that population-level behaviours may be well characterised by homogeneous ordinary differential equation models. Additionally, we demonstrate that population-level data are insufficient to distinguish resistance as a discrete phenotype from a continuous distribution of phenotypes. Our results inform the design of both future experiments and future quantitative analyses that probe adaptive resistance in cancer.
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Dates et versions

hal-04854906 , version 1 (24-12-2024)

Identifiants

  • HAL Id : hal-04854906 , version 1

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Alexander P Browning, Rebecca M Crossley, Chiara Villa, Philip K Maini, Adrianne L Jenner, et al.. Identifiability of heterogeneous phenotype adaptation from low-cell-count experiments and a stochastic model. 2024. ⟨hal-04854906⟩
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