In this paper, we establish a necessary and sufficient stability condition for a class of two coupled first-order linear hyperbolic partial differential equations. Through a backstepping transform, the problem is reformulated as a stability problem for an integral difference equation, that is, a difference equation with distributed delay. Building upon a Stépán–Hassard argument variation theorem originally designed for time-delay systems of retarded type, we then introduce a theorem that counts the number of unstable roots of our integral difference equation. This leads to the expected necessary and sufficient stability criterion for the system of first-order linear hyperbolic partial differential equations. Finally, we validate our theoretical findings through simulations.