This article aims to investigate the Ultra-High Frequency Dielectrophoresis technique to characterize populations of human mesenchymal stem cells. The principle is based on studying the displacement of a cell in an electric field gradient to quantify a characteristic frequency that establishes the UHF dielectrophoretic 'signature' of the investigated cell population. Preliminary results obtained from patient-derived cultures, in correlation with conventional biological analyses, seem to indicate that the osteogenic differentiation of mesenchymal stem cells can be detected by UHF-DEP, making this technique an interesting approach to characterize hMSC differentiation in the context of optimizing bioceramics for bone regenerative medicine.