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Poster De Conférence Année : 2023

Transgenerational neuroprotection of vegetal extract in neonatal hypoxia-ischemia

Résumé

Transgenerational neuroprotection of vegetal extract in neonatal hypoxia-ischemia Goudeneche P.1, Guichard C.1, Sanchez S.1, Beauvieux M-C.1, Chateil J-F., Pellerin L.2, Romain C.3, Cases J.3, Bouzier-Sore A-K.1, Roumes H.1 1 CRMSB - UMR 5536 - CNRS/University of Bordeaux - Bordeaux, France 2 IRMETIST - Inserm U1313, CHU/University of Poitiers - Poitiers, France 3 S.A.S. Fytexia - Vendres – FRANCE Introduction and purpose: Therapeutic strategy in neonatal hypoxia-ischemia (NHI), a major cause of perinatal death and chronic disability, is limited to hypothermia. As many newborns do not respond to it, new treatments are needed. Intraperitoneal injection of resveratrol (RSV, polyphenol present in grapes) has shown neuroprotective properties in NHI-pups but the doses used were high (20-100 mg/kg), the i.p. has a proinflammatory character and RSV has a low bioavailability (<1%). In order to overcome these limitations, we evaluated the effects of maternal supplementation with VE (vegetal extract rich in polyphenol), in the context of NHI. Material and methods: NHI (left common carotid artery ligation + hypoxia (8% O2, 92% N2, 2h) was induced in Wistar P7 rats (7 days post-natal). Maternal drinking water was supplemented with EV (0.15 mg/kg eq RSV), during the two weeks preceding the NHI event (EV group, n=16) or not supplemented (sham group, n=11 and Ct group, n=16). Brain lesion volumes (BLV) were measured in vivo, 3h (P7), 48h (P9) and 23d (P30) after NHI, using diffusion-weighted MRI (4.7T Bruker, TE=24ms, TR=2s, 30 directions, 20 sections, 1mm thick) and expressed as % of total brain volume. The severity of edema was assessed by measuring the apparent diffusion coefficient (ADC). Finally, motor and cognitive abilities were evaluated by behavioral tests. Results: NHI induced brain lesions in 100% of the Ct group (BLV, at P7: 46 ± 1%), and 56% of the EV group (BLV, at P7: 17 ± 5 %). Cytotoxic edema was significantly lower in the EV group (higher ADC values compared to the Ct group, p<0.001). At P30, 79% of the Ct group still had cerebral lesions compared to only 13% in the EV (BLV at P30: 22 ± 3 % vs 2 ± 2 %, for the groups HIC and EV, respectively. For behavioral tests, EV group recovered motor and cognitive abilities comparable to sham group. Conclusion: This study shows for the first time a neuroprotective role of EV maternal supplementation in the context of NHI. A nutritionally realistic daily amount was sufficient to avoid brain damage in some pups and to recover cognitive and motor functions. 1.Arteaga O. et al. PLoS One. 2015;10(11):e0142424. 2.Karalis F. et al., Brain Res. 2011;1425:98-110.
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hal-04632775 , version 1 (02-07-2024)

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  • HAL Id : hal-04632775 , version 1

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Pierre Goudeneche, Camille Guichard, Stéphane Sanchez, M-C Beauvieux, J-F Chateil, et al.. Transgenerational neuroprotection of vegetal extract in neonatal hypoxia-ischemia. ESMRMB, Oct 2023, Bâle (Suisse), Switzerland. ⟨hal-04632775⟩
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