Rose Bengal coupled to AGuIX NPs for anti-cancer photodynamic therapy
Résumé
Rose Bengal is a well-known photosensitizer used in the treatment of several diseases [1, 2]. This photosensitizer produces a large amount of singlet oxygen upon specific illumination, which allows its use in photodynamic therapy [3]. The work to be presented highlights the passive tumor targeting by coupling Rose Bengal to nanoparticles in clinical phase 2 AGuIX chelated with lanthanides (Terbium or Gadolinium).
One of the limitations of photodynamic therapy is the poor penetration of light into the tissues [4]. X-ray excitation of AGuIX(Ln)@RB overcomes this problem. Following the excitation of the lanthanide by X-ray, a FRET energy transfer from lanthanide to Rose Bengal takes place. Rose Bengal is then able to produce singlet oxygen. To enhance the targeting, peptide can be covalently couple to the nanoparticles [5]. Two types of peptide targeting NRP1 receptors over-expressed in neovessels were grafted on the nanoparticles.
This presentation focuses on the synthesis, photophysical properties of these nanoparticles, physical characterization, the type of energy transfer between the species, and the in vitro PDT-X effect.