Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue (Article De Synthèse) Journal of Cereal Science Année : 2012

Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI)

Eija Chiaramonte
  • Fonction : Auteur
L Rhazi
  • Fonction : Auteur
Thierry Aussenac
D Richard White
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Résumé

The rheological and functional properties of starch are influenced by the size and molar mass distribution of the polymer, the ratio of amylose (AMY) to amylopectin (AMP), and branching characteristics. Asymmetric Flow Field-Flow Fractionation (AF4) was applied to fractionate five different maize hybrids of varying AMY:AMP ratio. When coupled to detection by multi-angle light scattering and refractive index (MALSeRI), it was possible to determine mass percentage and the average weight-average molar mass (M w) without the need for calibration standards. Sufficient resolution of amylose and amylopectin was achieved by applying a gradient cross-flow on a 17 cm trapezoidal channel with a 350 mm spacer. The observed M w ranged from about 2 Â 10 5 to 4 Â 10 5 for amylose and from 1 Â 10 8 to 4 Â 10 8 for amylopectin. The corresponding z-average root-mean-square radii (R z) for AMP ranged from 145 to over 300 nm. Low recoveries from the AF4 channel were found to be due primarily to the focusing step. The calculated mass percent of AMY and AMP from integrated RI peak areas agreed well with nominal values for the individual starch hybrids. Both qualitative and quantitative data were reproducible. The results show the AF4eMALSeRI method to be well suited for routine molecular characterization of starch.
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hal-04310654 , version 1 (29-04-2024)

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Eija Chiaramonte, L Rhazi, Thierry Aussenac, D Richard White. Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI). Journal of Cereal Science, 2012, 56, pp.457 - 463. ⟨10.1016/j.jcs.2012.04.006⟩. ⟨hal-04310654⟩
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