g-NK cells from umbilical cord blood are phenotypically and functionally different than g-NK cells from peripheral blood - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue OncoImmunology Année : 2023

g-NK cells from umbilical cord blood are phenotypically and functionally different than g-NK cells from peripheral blood

Résumé

FcRγ-deficient natural killer (NK) cells, designated as g-NK cells, exhibit enhanced antibody-dependent cellular cytotoxicity (ADCC) capacity and increased IFN-γ and TNF-α production, rendering them promising for antiviral and antitumor responses. g-NK cells from peripheral blood (PB) are often associated with prior human cytomegalovirus (HCMV) infection. However, the prevalence, phenotype, and function of g-NK cells in umbilical cord blood (UCB-g-NK) remain unclear. Here, we demonstrate significant phenotypical differences between UCB-g-NK and PB-g-NK cells. Unlike PB-g-NK cells, UCB-g-NK cells did not show heightened cytokine production upon CD16 engagement, in contrast to the conventional NK (c-NK) cell counterparts. Interestingly, following in vitro activation, UCB-g-NK cells also exhibited elevated levels of IFN-γ production, particularly when co-cultured with HCMV and plasma from g-NK + adults. Furthermore, g-NK + plasma from PB even facilitated the in vitro expansion of UCB-g-NK cells. These findings underscore the phenotypic and functional heterogeneity of g-NK cells based on their origin and demonstrate that components within g-NK + plasma may directly contribute to the acquisition of an adult phenotype by the "immature" UCB-g-NK cells.
Fichier principal
Vignette du fichier
Gao_al_Oncoimmunology_2023.pdf (3.48 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

hal-04301529 , version 1 (23-11-2023)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Fei Gao, Mauricio Campos-Mora, Michael Constantinides, Loïs Coenon, Caroline Multrier, et al.. g-NK cells from umbilical cord blood are phenotypically and functionally different than g-NK cells from peripheral blood. OncoImmunology, 2023, 12 (1), pp.2283353. ⟨10.1080/2162402x.2023.2283353⟩. ⟨hal-04301529⟩

Collections

UNIV-MONTPELLIER
3 Consultations
9 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More