This work deals with the non-cutoff Boltzmann equation for all type of potentials, in both the torus $\mathbf{T}^3$ and in the whole space $\mathbf{R}^3$, under the incompressible Navier-Stokes scaling. We first establish the well-posedness and decay of global mild solutions to this rescaled Boltzmann equation in a perturbative framework, that is for solutions close to the Maxwellian, obtaining in particular integrated-in-time regularization estimates. We then combine these estimates with spectral-type estimates in order to obtain the strong convergence of solutions to the non-cutoff Boltzmannn equation towards the incompressible Navier-Stokes-Fourier system.