Validation of quantitative real-time polymerase chain reaction for detection of Legionella pneumophila in hospital water networks
Validation of quantitative real-time polymerase chain reaction for detection of Legionella pneumophila in hospital water networks.
Résumé
Rapid monitoring of Legionella pneumophila (Lp) is essential to reduce the risk of Legionnaires' disease in healthcare facilities. However, culture results take at least eight days, delaying the implementation of corrective measures. Here, we assessed the performance of a qPCR method and determined qPCR action thresholds for the detection of Lp in hospital hot water networks (HWNs).