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Article Dans Une Revue Polyhedron Année : 2019

New homochiral and heterochiral Mo(VI) complex from racemic ligand: Synthesis, X-ray structure, diastereomers separation and biological activities

Résumé

A diastereomeric pair (homochiral and heterochiral) of Schiff base dioxo molybdenum complex was synthesized using chiral ligand (HL: R/S-1-((naphtalen-3-yl)methylenamino)propan-2-ol) in racemic form. Then, to separation of this diastereomeric pair, crystallization method was proposed which was successful with acetonitrile. 1H NMR technique was used to confirm the results. The complexes thoroughly characterized using FT-IR, elemental analysis, 1H NMR and 13C NMR techniques, and MoO2L(LH2)1 has been structurally characterized using single-crystal X-ray diffraction. The cytotoxic activity of the new compounds has been evaluated using MCF-7 (human breast adenocarcinoma) and HeLa (human cervix adenocarcinoma), in addition to normal human fibroblast cells using the MTT cytotoxicity assay. Compounds MoO2L(LH2)1 and MoO2L(LH2)2 revealed IC50 values 18 µM and 37 µM on MCF-7 and 58 µM and 17 µM on HeLa, respectively. MoO2L(LH2)2 showed high selectivity (3–13 folds) for cancerous cells over normal cells, as the maximum cell mortality of 8.77%. Furthermore, The HSA- and DNA-binding of the Mo(VI) complexes were investigated by absorption, emission spectroscopy, viscosity measurements and molecular docking. Their binding constant are calculated as: HSA-binding of MoO2L(LH2)1 is 6.7 × 104 M−1 and of MoO2L(LH2)2 is 5.8 × 104 M−1, while their DNA-binding are 5.6 × 104 M−1 and 2.2 × 105 M−1, respectively. Their thermodynamic parameters were also determined at different temperatures.
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hal-04085385 , version 1 (28-04-2023)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Zahra Kazemi, Hadi Amiri Rudbari, Mehdi Sahihi, Valiollah Mirkhani, Majid Moghadam, et al.. New homochiral and heterochiral Mo(VI) complex from racemic ligand: Synthesis, X-ray structure, diastereomers separation and biological activities. Polyhedron, 2019, 170, pp.70-85. ⟨10.1016/j.poly.2019.05.021⟩. ⟨hal-04085385⟩
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