The SELENOT mimetic PSELT promotes nerve regeneration by increasing axonal myelination in a facial nerve injury model in female rats - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Neuroscience Research Année : 2022

The SELENOT mimetic PSELT promotes nerve regeneration by increasing axonal myelination in a facial nerve injury model in female rats

Hugo Pothion
  • Fonction : Auteur
Isabelle Lihrmann
  • Fonction : Auteur
Celia Duclos
  • Fonction : Auteur
Gaëtan Riou
  • Fonction : Auteur
Dorthe Cartier
  • Fonction : Auteur
Loubna Boukhzar
  • Fonction : Auteur
Benjamin Lefranc
  • Fonction : Auteur
Nicolas Guérout
Jean‐paul Marie
  • Fonction : Auteur
Youssef Anouar
  • Fonction : Auteur

Résumé

Introduction: The aim of the study is to investigate whether acute or chronic central administration of the hypothalamic neuropeptide 26RFa may ameliorate the glycemic control of obese/diabetic mice. Methods: Mice were treated for 4 months with a high-fat (HF) diet and received a single i.c.v. injection of 26RFa (3 µg) or a chronic i.c.v. administration of the peptide during 28 days via osmotic minipumps (25 µg/day). i.p. and oral glucose (GLU) tolerance tests, insulin (INS) tolerance test, glucose-stimulated insulin secretion (GSIS), food/water intake, horizontal/vertical activity, energy expenditure, meal pattern, and whole-body composition were monitored. In addition, 26RFa and GPR103 mRNA expressions as well as plasma 26RFa levels were evaluated by RT-QPCR and radioimmunoassay. Results: Acute administration of 26RFa in HF mice induced a robust antihyperglycemic effect by enhancing INS secretion, whereas chronic administration of the neuropeptide is unable to improve glucose homeostasis in these obese/diabetogenic conditions. By contrast, chronic 26RFa treatment induced an increase of the body weight accompanied with an enhanced food intake and a decreased energy expenditure. Finally, we show that the HF diet does not alter the hypothalamic expression of the 26RFa/GPR103 neuropeptidergic system nor the levels of circulating 26RFa. Conclusion: Our data indicate that the central beneficial effect of 26RFa on glucose homeostasis, by potentiating GSIS, is preserved in HF mice. However, chronic administration of the neuropeptide is unable to balance glycemia in these pathophysiological conditions, suggesting that the hypothalamic 26RFa/GPR103 neuropeptidergic system mainly affects short-term regulation of glucose metabolism.

Dates et versions

hal-03936105 , version 1 (12-01-2023)

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Citer

Hugo Pothion, Isabelle Lihrmann, Celia Duclos, Gaëtan Riou, Dorthe Cartier, et al.. The SELENOT mimetic PSELT promotes nerve regeneration by increasing axonal myelination in a facial nerve injury model in female rats. Journal of Neuroscience Research, 2022, 100 (9), pp.1721-1731. ⟨10.1002/jnr.25098⟩. ⟨hal-03936105⟩
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