Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert - Archive ouverte HAL
Article Dans Une Revue Antibiotics Année : 2022

Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert

Raoua Ben Brahim
  • Fonction : Auteur
Hasna Ellouzi
  • Fonction : Auteur
Khaoula Fouzai
  • Fonction : Auteur
Nedra Asses
  • Fonction : Auteur
Mohammed Neffati
  • Fonction : Auteur
Jean Marc Sabatier
Philippe Bulet
Université Grenoble Alpes
Imed Regaya
  • Fonction : Auteur

Résumé

Extraction methods depend mainly on the chemical nature of the extracted molecule. For these reasons, the selection of the extraction medium is a vital part of obtaining these molecules. The extraction of antimicrobial peptides (AMPs) from extremophile plants is important because of its potential pharmaceutical applications. This work focused on the evaluation of several solvents for the extraction of AMPs from the following two extremophile plants: Astragalus armatus and Anthyllis sericea from southern Tunisia. In order to identify the most efficient solvents and extraction solutions, we used sulfuric acid, dichloromethane, phosphate buffer, acetic acid and sodium acetate, and we tested them on leaves and roots of both the studied plants. The extracts obtained using sulfuric acid, dichloromethane and phosphate buffer extraction did not show any antimicrobial activity, whereas the acetic acid and sodium acetate extracts led to growth inhibition of some of the tested bacterial strains. The extracts of leaves and roots of An. sericea and As. armatus obtained by acetic acid and sodium acetate were proven to be active against Gram-positive bacteria and Gram-negative bacteria. Therefore, the most appropriate solvents to use for antimicrobial peptide extraction from both plants are acetic acid and sodium acetate.

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Sciences du Vivant [q-bio]

Philippe BULET  : Connectez-vous pour contacter le contributeur

https://hal.science/hal-03843746

Soumis le : mardi 8 novembre 2022-13:01:44

Dernière modification le : mercredi 18 décembre 2024-09:36:11

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hal-03843746 , version 1 (08-11-2022)

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Raoua Ben Brahim, Hasna Ellouzi, Khaoula Fouzai, Nedra Asses, Mohammed Neffati, et al.. Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert. Antibiotics, 2022, 11 (10), pp.1302. ⟨10.3390/antibiotics11101302⟩. ⟨hal-03843746⟩

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