<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03843746</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T05:42:45+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert</title>
            <author role="aut">
              <persName>
                <forename type="first">Raoua</forename>
                <surname>Ben Brahim</surname>
              </persName>
              <idno type="halauthorid">2595107-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Hasna</forename>
                <surname>Ellouzi</surname>
              </persName>
              <idno type="halauthorid">1197547-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Khaoula</forename>
                <surname>Fouzai</surname>
              </persName>
              <idno type="halauthorid">2595108-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nedra</forename>
                <surname>Asses</surname>
              </persName>
              <idno type="halauthorid">2595109-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Mohammed</forename>
                <surname>Neffati</surname>
              </persName>
              <idno type="halauthorid">2595110-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jean Marc</forename>
                <surname>Sabatier</surname>
              </persName>
              <email type="md5">a24fd7ad37a82aa9aa3b40e3ce1707c1</email>
              <email type="domain">gmail.com</email>
              <idno type="idhal" notation="string">sabatierjm</idno>
              <idno type="idhal" notation="numeric">178878</idno>
              <idno type="halauthorid" notation="string">2595111-178878</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-9040-5647</idno>
              <idno type="IDREF">https://www.idref.fr/034150722</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Philippe</forename>
                <surname>Bulet</surname>
              </persName>
              <email type="md5">6e8b33dbdb4816a45bc68a232f851d79</email>
              <email type="domain">univ-grenoble-alpes.fr</email>
              <idno type="idhal" notation="string">philippe-bulet</idno>
              <idno type="idhal" notation="numeric">180758</idno>
              <idno type="halauthorid" notation="string">4700-180758</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9016-265X</idno>
              <idno type="IDREF">https://www.idref.fr/128857250</idno>
              <affiliation ref="#struct-1042703"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Imed</forename>
                <surname>Regaya</surname>
              </persName>
              <idno type="halauthorid">388238-0</idno>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Philippe</forename>
                <surname>BULET</surname>
              </persName>
              <email type="md5">6e8b33dbdb4816a45bc68a232f851d79</email>
              <email type="domain">univ-grenoble-alpes.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-11-08 13:01:44</date>
              <date type="whenModified">2025-09-27 19:23:44</date>
              <date type="whenReleased">2022-11-08 13:01:44</date>
              <date type="whenProduced">2022-09-24</date>
              <ref type="externalLink" target="https://www.mdpi.com/2079-6382/11/10/1302/pdf?version=1664190729"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="815856">
                <persName>
                  <forename>Philippe</forename>
                  <surname>BULET</surname>
                </persName>
                <email type="md5">6e8b33dbdb4816a45bc68a232f851d79</email>
                <email type="domain">univ-grenoble-alpes.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03843746</idno>
            <idno type="halUri">https://hal.science/hal-03843746</idno>
            <idno type="halBibtex">benbrahim:hal-03843746</idno>
            <idno type="halRefHtml">&lt;i&gt;Antibiotics&lt;/i&gt;, 2022, 11 (10), pp.1302. &lt;a target="_blank" href="https://dx.doi.org/10.3390/antibiotics11101302"&gt;&amp;#x27E8;10.3390/antibiotics11101302&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Antibiotics, 2022, 11 (10), pp.1302. &amp;#x27E8;10.3390/antibiotics11101302&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="UGA-EPE">Université Grenoble Alpes [2020-*]</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Optimized Chemical Extraction Methods of Antimicrobial Peptides from Roots and Leaves of Extremophilic Plants: Anthyllis sericea and Astragalus armatus Collected from the Tunisian Desert</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Raoua</forename>
                    <surname>Ben Brahim</surname>
                  </persName>
                  <idno type="halauthorid">2595107-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Hasna</forename>
                    <surname>Ellouzi</surname>
                  </persName>
                  <idno type="halauthorid">1197547-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Khaoula</forename>
                    <surname>Fouzai</surname>
                  </persName>
                  <idno type="halauthorid">2595108-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nedra</forename>
                    <surname>Asses</surname>
                  </persName>
                  <idno type="halauthorid">2595109-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Mohammed</forename>
                    <surname>Neffati</surname>
                  </persName>
                  <idno type="halauthorid">2595110-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jean Marc</forename>
                    <surname>Sabatier</surname>
                  </persName>
                  <email type="md5">a24fd7ad37a82aa9aa3b40e3ce1707c1</email>
                  <email type="domain">gmail.com</email>
                  <idno type="idhal" notation="string">sabatierjm</idno>
                  <idno type="idhal" notation="numeric">178878</idno>
                  <idno type="halauthorid" notation="string">2595111-178878</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-9040-5647</idno>
                  <idno type="IDREF">https://www.idref.fr/034150722</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Philippe</forename>
                    <surname>Bulet</surname>
                  </persName>
                  <email type="md5">6e8b33dbdb4816a45bc68a232f851d79</email>
                  <email type="domain">univ-grenoble-alpes.fr</email>
                  <idno type="idhal" notation="string">philippe-bulet</idno>
                  <idno type="idhal" notation="numeric">180758</idno>
                  <idno type="halauthorid" notation="string">4700-180758</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9016-265X</idno>
                  <idno type="IDREF">https://www.idref.fr/128857250</idno>
                  <affiliation ref="#struct-1042703"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Imed</forename>
                    <surname>Regaya</surname>
                  </persName>
                  <idno type="halauthorid">388238-0</idno>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">22688</idno>
                <idno type="eissn">2079-6382</idno>
                <title level="j">Antibiotics</title>
                <imprint>
                  <publisher>MDPI</publisher>
                  <biblScope unit="volume">11</biblScope>
                  <biblScope unit="issue">10</biblScope>
                  <biblScope unit="pp">1302</biblScope>
                  <date type="datePub">2022-09-24</date>
                </imprint>
              </monogr>
              <idno type="doi">10.3390/antibiotics11101302</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>&lt;jats:p&gt;Extraction methods depend mainly on the chemical nature of the extracted molecule. For these reasons, the selection of the extraction medium is a vital part of obtaining these molecules. The extraction of antimicrobial peptides (AMPs) from extremophile plants is important because of its potential pharmaceutical applications. This work focused on the evaluation of several solvents for the extraction of AMPs from the following two extremophile plants: Astragalus armatus and Anthyllis sericea from southern Tunisia. In order to identify the most efficient solvents and extraction solutions, we used sulfuric acid, dichloromethane, phosphate buffer, acetic acid and sodium acetate, and we tested them on leaves and roots of both the studied plants. The extracts obtained using sulfuric acid, dichloromethane and phosphate buffer extraction did not show any antimicrobial activity, whereas the acetic acid and sodium acetate extracts led to growth inhibition of some of the tested bacterial strains. The extracts of leaves and roots of An. sericea and As. armatus obtained by acetic acid and sodium acetate were proven to be active against Gram-positive bacteria and Gram-negative bacteria. Therefore, the most appropriate solvents to use for antimicrobial peptide extraction from both plants are acetic acid and sodium acetate.&lt;/jats:p&gt;</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="regroupinstitution" xml:id="struct-1042703" status="VALID">
          <idno type="IdRef">240648315</idno>
          <idno type="ROR">https://ror.org/02rx3b187</idno>
          <orgName>Université Grenoble Alpes</orgName>
          <orgName type="acronym">UGA</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>Adresse CS 40700 - 38058 Grenoble cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-grenoble-alpes.fr</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>