Lab-on-a-Chip for the isolation of circulating Adipose Stem Cells - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2022

Lab-on-a-Chip for the isolation of circulating Adipose Stem Cells

Résumé

Adipose stem cells (ASC) are relatively abundant multipotent stem cells dispersed in fat tissue in the body. They could be considered as diabetes biomarkers since studies showed that they are released by subcutaneous adipose tissue during type 2 diabetes development and are supposed to circulate in blood. However detecting circulating ASCs is rather difficult since they do not express specific unique membrane markers compared to other hematopoietic cells, which renders their isolation from whole blood a hard task. In this work, we aimed to separate ASC from whole peripheral blood, using a setup combining two complementary depletion microfluidic modules. The first one is a hydrodynamic filtration module that sorts particles according to their diameter (Rc = 10 μm) and which therefore will eliminate small cells as red blood cells and platelets. The second one is a “cell rolling” module that deplete remaining leucocytes according to their surface antigens, leading to a sample that ideally contains only ASC.
Fichier principal
Vignette du fichier
GDR MNF-B2I 2022.pdf (2.46 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03823431 , version 1 (20-10-2022)

Identifiants

  • HAL Id : hal-03823431 , version 1

Citer

Mohammad Hussein Baz, Lucas Charpin, Audrey Varin, Coralie Sengenès, Emmanuelle Trevisiol, et al.. Lab-on-a-Chip for the isolation of circulating Adipose Stem Cells. GDR Micro Nanofluidics GDR Bio ingénierie des Interfaces, Oct 2022, Paris, France. 2022. ⟨hal-03823431⟩
71 Consultations
16 Téléchargements

Partager

Gmail Facebook X LinkedIn More