Adipose stem cells (ASC) are relatively abundant multipotent stem cells dispersed in fat tissue in the body. They could be considered as diabetes biomarkers since studies showed that they are released by subcutaneous adipose tissue during type 2 diabetes development and are supposed to circulate in blood. However detecting circulating ASCs is rather difficult since they do not express specific unique membrane markers compared to other hematopoietic cells, which renders their isolation from whole blood a hard task. In this work, we aimed to separate ASC from whole peripheral blood, using a setup combining two complementary depletion microfluidic modules. The first one is a hydrodynamic filtration module that sorts particles according to their diameter (Rc = 10 μm) and which therefore will eliminate small cells as red blood cells and platelets. The second one is a “cell rolling” module that deplete remaining leucocytes according to their surface antigens, leading to a sample that ideally contains only ASC.