Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Photonics Année : 2022

Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy

Résumé

Multi-photon microscopy has played a significant role in biological imaging since it allows to observe living tissues with improved penetration depth and excellent sectioning effect. Multi-photon microscopy relies on multi-photon absorption, enabling the use of different imaging modalities that strongly depends on the properties of the sample structure, the selected fluorophore and the excitation laser. However, versatile and tunable laser excitation for multi-photon absorption is still a challenge, limited by e.g. the narrow bandwidth of typical laser gain medium or by the tunability of wavelength conversion offered by optical parametric oscillators or amplifiers. As an alternative, supercontinuum generation can provide broadband excitations spanning from the ultra-violet to far infrared domains and integrating numerous fluorophore absorption peaks, in turn enabling different imaging modalities or potential multiplexed spectroscopy. Here, we report on the use of machine learning to optimize the spectro-temporal properties of supercontinuum generation in order to selectively enhance multi-photon excitation signals compatible with a variety of fluorophores (or modalities) for multi-photon microscopy. Specifically, we numerically explore how the use of reconfigurable (femtosecond) pulse patterns can be readily exploited to control the nonlinear propagation dynamics and associated spectral broadening occurring in a highly-nonlinear fiber. In this framework, we show that the use of multiple pulses to seed optical fiber propagation can trigger a variety of nonlinear interactions and complex propagation scenarios. This approach, exploiting the temporal dimension as an extended degree of freedom, is used to maximize typical multi-photon excitations at selected wavelengths, here obtained in a versatile and reconfigurable manner suitable for imaging applications. We expect these results to pave the way towards on-demand and real time supercontinuum shaping, with further multi-photon microscopy improvements in terms of spatial 3D resolution, optical toxicity, and wavelength selectivity.
Fichier principal
Vignette du fichier
fphot-03-940902 (4).pdf (4.71 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03771437 , version 1 (07-09-2022)

Licence

Paternité

Identifiants

Citer

Van Thuy Hoang, Yassin Boussafa, Lynn Sader, Sébastien Février, Vincent Couderc, et al.. Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy. Frontiers in Photonics, 2022, 3, pp.940902. ⟨10.3389/fphot.2022.940902⟩. ⟨hal-03771437⟩
27 Consultations
29 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More