Development and validation according to the SANTE guidelines of a QuECh-ERS-UHPLC-QTOF-MS method for the screening of 204 pesticides in bivalves - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Food Chemistry Année : 2022

Development and validation according to the SANTE guidelines of a QuECh-ERS-UHPLC-QTOF-MS method for the screening of 204 pesticides in bivalves

Résumé

A qualitative screening high resolution mass spectrometry method was developed and validated according to the EU SANTE/12682/2019 guidelines for the analysis of 204 pesticides in seven commercial bivalve species spiked at three concentrations (0.01, 0.05 and, 0.1 mg.kg−1). Samples were extracted using QuEChERS and analysed using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The QuEChERS method was optimised by the Taguchi Orthogonal Array approach. The best conditions were obtained with pure ACN, MgSO4/NaCl as extraction salts, MgSO4/PSA/C18 as clean-up, and the non-dilution of extracts. The impact of different HRMS acquisition modes on detection and identification rates were also evaluated. The screening detection limits were determined to be 0.01 mg.kg−1 and 0.1 for 66% and 87% of pesticides, respectively. These screening procedure was finally applied to different bivalve samples using target and suspect analysis. This allowed the identification of diuron and its metabolite 1-(3,4-dichlorophenyl)-3-methylurea in the investigated samples
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Dates et versions

hal-03628271 , version 1 (01-04-2022)

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Thierno Diallo, Yassine Makni, Adélaïde Lerebours, Hélène Thomas, Thierry Guérin, et al.. Development and validation according to the SANTE guidelines of a QuECh-ERS-UHPLC-QTOF-MS method for the screening of 204 pesticides in bivalves. Food Chemistry, 2022, 386, pp.132871. ⟨10.1016/j.foodchem.2022.132871⟩. ⟨hal-03628271⟩
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