Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation - Archive ouverte HAL
Article Dans Une Revue Front.Genet. Année : 2021

Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation

Mamadou Soumboundou
  • Fonction : Auteur
Julien Dossou
  • Fonction : Auteur
Yossef Kalaga
  • Fonction : Auteur
Innocent Nkengurutse
  • Fonction : Auteur
Ibrahima Faye
Albert Guingani
  • Fonction : Auteur
Macoura Gadji
  • Fonction : Auteur
Koudbi J. Yameogo
  • Fonction : Auteur
Henri Zongo
  • Fonction : Auteur
Gora Mbaye
  • Fonction : Auteur
Ahmadou Dem
  • Fonction : Auteur
Mounibé Diarra
  • Fonction : Auteur
Rached Adjibade
  • Fonction : Auteur
Catherine Djebou
  • Fonction : Auteur
Steffen Junker
  • Fonction : Auteur
Noufissa Oudrhiri
  • Fonction : Auteur
William M. Hempel
  • Fonction : Auteur
Alain Dieterlen
  • Fonction : Auteur
Eric Jeandidier
  • Fonction : Auteur
Patrice Carde
  • Fonction : Auteur
Elie El Maalouf
  • Fonction : Auteur
Bruno Colicchio
  • Fonction : Auteur
Annelise Bennaceur-Griscelli
  • Fonction : Auteur
Michael Fenech
  • Fonction : Auteur
Philippe Voisin
  • Fonction : Auteur
Claire Rodriguez-Lafrasse
Institut de Physique des 2 Infinis de Lyon
Radhia M'Kacher
  • Fonction : Auteur

Résumé

Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry.Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0–4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations.Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10–17), centric rings (p = 4.0310–14), and resulting double-strand-breaks (DSB) (p = 1.32 10–18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa.Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.

Domaines

Physique [physics]

INSPIRE HEP  : Connectez-vous pour contacter le contributeur

https://hal.science/hal-03472634

Soumis le : jeudi 9 décembre 2021-14:15:09

Dernière modification le : jeudi 11 avril 2024-13:18:12

Consulter le texte intégral

Dates et versions

hal-03472634 , version 1 (09-12-2021)

Identifiants

Citer

Mamadou Soumboundou, Julien Dossou, Yossef Kalaga, Innocent Nkengurutse, Ibrahima Faye, et al.. Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation. Front.Genet., 2021, 12, pp.657999. ⟨10.3389/fgene.2021.657999⟩. ⟨hal-03472634⟩

Exporter

BibTeX XML-TEI Dublin Core DC Terms EndNote DataCite

Collections

IN2P3 CNRS UNIV-LYON1 PRIMES PRIMES_WP3 IP2I UDL CMS IPM PHABIO MANOIR THEORIE COSMOLOGIE MATNUC NEUTRINOS_IP2I ALICE_IP2I ONDES_GRAVITATIONNELLES MATICE
29 Consultations
0 Téléchargements

Altmetric

Partager

More