Development of a 3D Method Using SEM and Liquid-Phase STEM to Study Cell Morphology
Résumé
Cell adhesion is involved in many cellular processes such as proliferation, migration and differentiation. Cell adhesion conditions cell morphology, indeed recent studies have shown that the substrate on which cells are cultured can lead to different cell morphologies [1]. A challenging investigation related cell morphology and gene expression in response to nanotopography [2]. The morphological analysis is mostly done in 2D using high vacuum scanning electron microscopy (HVSEM). We aim to set a 3D analysis method using HVSEM and liquid-phase SEM (LPSEM) to reduce changes occurring during sample preparation, such as dehydration. A home-made scanning transmission electron microscopy
(STEM) tomography device, specific to LPSEM, has previously been developed to study aqueous suspensions in 3D [3].
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