Gelatin is a fascinating biomaterial due to its biocompatibility, biodegradability and chemical composition. The engineering of gelatin scaffolds with the most appropriate architecture is crucial for tissue engineering applications. Currently available porogen assisted scaffold fabrication technique are limited for the fabrication of repeatable and customized scaffolds. Three dimensional printing is an appropriate technique to prepare customized structure. In this report, customized gelatin scaffolds were fabricated using 3D printed polyvinyl alcohol (PVA) sacrificial molds. Gelatin was stabilized through crosslinking and the PVA was solubilized to obtain 3D customized gelatin scaffolds. The gelatin scaffolds displayed tunable pore size from 350 to 1200 ​μm. The influence of PVA infill density on the gelatin scaffold porous structure and interconnectivity was also analyzed. Furthermore, the gelatin scaffold stability in phosphate buffered saline solution and their swelling capacity demonstrated that they were effectively crosslinked. The scaffold cytocompatibility and the good cell attachment and proliferation on these scaffolds showed that the sacrificial mold-assisted 3D printing is suitable for producing customizable gelatin scaffolds for biomedical applications.