Terbium-based AGuIX-design nanoparticle to mediate X-ray-induced photodynamic therapy - Archive ouverte HAL Access content directly
Journal Articles Pharmaceuticals Year : 2021

Terbium-based AGuIX-design nanoparticle to mediate X-ray-induced photodynamic therapy

Joël Daouk
  • Function : Author
  • PersonId : 1082537
Mathilde Iltis
  • Function : Author
  • PersonId : 1096804
Batoul Dhaini
  • Function : Author
  • PersonId : 1082538
Denise Béchet
  • Function : Author
  • PersonId : 1096805
Alain Delconte
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  • PersonId : 1066546
  • IdRef : 139793623
Benoît Habermeyer
  • Function : Author
  • PersonId : 1096807
Hervé Schohn
  • Function : Author
  • PersonId : 1061662


X-ray-induced photodynamic therapy is based on the energy transfer from a nanoscintillator to a photosensitizer molecule, whose activation leads to singlet oxygen and radical species generation, triggering cancer cells to cell death. Herein, we synthesized ultra-small nanoparticle chelated with Terbium (Tb) as a nanoscintillator and 5-(4-carboxyphenyl succinimide ester)-10,15,20-triphenyl porphyrin (P1) as a photosensitizer (AGuIX@Tb-P1). The synthesis was based on the AGuIX@ platform design. AGuIX@Tb-P1 was characterised for its photo-physical and physico-chemical properties. The effect of the nanoparticles was studied using human glioblastoma U-251 MG cells and was compared to treatment with AGuIX@ nanoparticles doped with Gadolinium (Gd) and P1 (AguIX@Gd-P1). We demonstrated that the AGuIX@Tb-P1 design was consistent with X-ray photon energy transfer from Terbium to P1. Both nanoparticles had similar dark cytotoxicity and they were absorbed in a similar rate within the cells. Pre-treated cells exposure to X-rays was related to reactive species production. Using clonogenic assays, establishment of survival curves allowed discrimination of the impact of radiation treatment from X-ray-induced photodynamic effect. We showed that cell growth arrest was increased (35%-increase) when cells were treated with AGuIX@Tb-P1 compared to the nanoparticle doped with Gd
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hal-03206070 , version 1 (22-04-2021)



Joël Daouk, Mathilde Iltis, Batoul Dhaini, Denise Béchet, Philippe Arnoux, et al.. Terbium-based AGuIX-design nanoparticle to mediate X-ray-induced photodynamic therapy. Pharmaceuticals, 2021, 14 (5), pp.396. ⟨10.3390/ph14050396⟩. ⟨hal-03206070⟩
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