Validation of a three-dimensional head and neck spheroid model to evaluate cameras for NIR fluorescence-guided cancer surgery - Archive ouverte HAL Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2021

Validation of a three-dimensional head and neck spheroid model to evaluate cameras for NIR fluorescence-guided cancer surgery

Abstract

Near-infrared (NIR) fluorescence-guided surgery is an innovative technique for the real-time visualization of resection margins. The aim of this study was to develop a head and neck multicellular tumor spheroid model and to explore the possibilities offered by it for the evaluation of cameras for NIR fluorescence-guided surgery protocols. FaDu spheroids were incubated with indocyanine green (ICG) and then included in a tissue-like phantom. To assess the capability of Fluobeam® NIR camera to detect ICG in tissues, FaDu spheroids exposed to ICG were embedded in 2, 5 or 8 mm of tissue-like phantom. The fluorescence signal was significantly higher between 2, 5 and 8 mm of depth for spheroids treated with more than 5 µg/mL ICG (p < 0.05). The fluorescence intensity positively correlated with the size of spheroids (p < 0.01), while the correlation with depth in the tissue-like phantom was strongly negative (p < 0.001). This multicellular spheroid model embedded in a tissue-like phantom seems to be a simple and reproducible in vitro tumor model, allowing a comparison of NIR cameras. The ideal configuration seems to be 450 μm FaDu spheroids incubated for 24 hours with 0.05 mg/ml of ICG, ensuring the best stability, toxicity, incorporation and signal intensity.
Fichier principal
Vignette du fichier
ijms-22-01966.pdf (2.34 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03162289 , version 1 (25-10-2023)

Licence

Attribution

Identifiers

Cite

Claire Egloff-Juras, Ilya Yakavets, Victoria Scherrer, Aurélie Francois, Lina Bezdetnaya, et al.. Validation of a three-dimensional head and neck spheroid model to evaluate cameras for NIR fluorescence-guided cancer surgery. International Journal of Molecular Sciences, 2021, 22 (4), pp.1966. ⟨10.3390/ijms22041966⟩. ⟨hal-03162289⟩
52 View
1 Download

Altmetric

Share

Gmail Facebook X LinkedIn More