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Article Dans Une Revue Stem Cell Research Année : 2014

Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies

Mélanie Lavergne
  • Fonction : Auteur
  • PersonId : 1087498
Elisa Negroni
Ségolène Ferratge
  • Fonction : Auteur
  • PersonId : 1087499
Gilles Carpentier
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  • PersonId : 1076198
Marie Gilbert-Sirieix
  • Fonction : Auteur
Fernando Siñeriz
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  • PersonId : 1087500
Georges Uzan
Patricia Albanese

Résumé

Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, it is difficult to reach the cell dose required for cell therapy of vascular diseases, thus limiting the clinical use of these cells. Glycosaminoglycans (GAG) are components from the extracellular matrix (ECM) that are able to interact and potentiate heparin binding growth factor (HBGF) activities. According to these relevant biological properties of GAG, we designed a GAG mimetic having the capacity to increase the yield of ECFC production from blood and to improve functionality of their endothelial outgrowth. We demonstrate that the addition of [OTR 4131 ] mimetic during the isolation process of ECFC from Cord Blood induces a 3 fold increase in the number of colonies. Moreover, addition of [OTR 4131 ] to cell culture media improves adhesion, proliferation, migration and self-renewal of ECFC. We provide evidence showing that GAG mimetics may have great interest for cell therapy applied to vascular regeneration therapy and represent an alternative to exogenous growth factor treatments to optimize potential therapeutic properties of ECFC.
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Dates et versions

hal-03097738 , version 1 (05-01-2021)

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Fabien Chevalier, Mélanie Lavergne, Elisa Negroni, Ségolène Ferratge, Gilles Carpentier, et al.. Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies. Stem Cell Research, 2014, 12 (3), pp.703-715. ⟨10.1016/j.scr.2014.03.001⟩. ⟨hal-03097738⟩
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