PB-94 [14:30] Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells
Résumé
This study looks at the application of genetically encoded calcium indicators (GECIs), to investigate the spatiotemporal nature of nsPEF-induced calcium signals, using fluorescent live cell imaging. GECIs can be targeted to different cellular locations and, by using a plasma membrane expressed GECI, we demonstrate calcium entry into the cell as a wave, initiated at the cathode side, following nsPEF treatment. This wave was masked by commercially available chemical, cytosolic, calcium indicators.
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