PB-94 [14:30] Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2018

PB-94 [14:30] Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells

Lynn Carr
  • Fonction : Auteur
Sylvia M. Bardet
Rodney P O'Connor
  • Fonction : Auteur

Résumé

This study looks at the application of genetically encoded calcium indicators (GECIs), to investigate the spatiotemporal nature of nsPEF-induced calcium signals, using fluorescent live cell imaging. GECIs can be targeted to different cellular locations and, by using a plasma membrane expressed GECI, we demonstrate calcium entry into the cell as a wave, initiated at the cathode side, following nsPEF treatment. This wave was masked by commercially available chemical, cytosolic, calcium indicators.

Mots clés

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Dates et versions

hal-03031490 , version 1 (30-11-2020)

Identifiants

  • HAL Id : hal-03031490 , version 1

Citer

Lynn Carr, Sylvia M. Bardet, Rodney P O'Connor. PB-94 [14:30] Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells. BioEM2018, 2018, Portoroz, France. 2018. ⟨hal-03031490⟩

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