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Article Dans Une Revue Nature Catalysis Année : 2019

A family of native amine dehydrogenases for the asymmetric reductive amination of ketones

Jean-Louis Petit
Aline Mariage
Alain Perret
Véronique de Berardinis
Anne Zaparucha
Gideon Grogan
  • Fonction : Auteur
  • PersonId : 870289
Carine Vergne-Vaxelaire

Résumé

The asymmetric reductive amination of ketones enables the one-step synthesis of chiral amines from readily available starting materials. Here we report the discovery of a family of native NAD(P)H-dependent amine dehydrogenases (nat-AmDHs) competent for the asymmetric reductive amination of aliphatic and alicyclic ketones, adding significantly to the biocatalytic toolbox available for chiral amine synthesis. Studies of ketone and amine substrate specificity and kinetics reveal a strong preference for aliphatic ketones and aldehydes, with activities of up to 614.5 mU mg−1 for cyclohexanone with ammonia, and 851.3 mU mg−1 for isobutyraldehyde with methylamine as the amine donor. Crystal structures of three nat-AmDHs (AmDH4, MsmeAmDH and CfusAmDH) reveal the active site determinants of substrate and cofactor specificity and enable the rational engineering of AmDH4 for the generated activity towards pentan-2-one. Analysis of the three-dimensional catalytic site distribution among bacterial biodiversity revealed a superfamily of divergent proteins with representative specificities ranging from amino acid substrates to hydrophobic ketones.
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Dates et versions

hal-02945525 , version 1 (22-09-2020)

Identifiants

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Ombeline Mayol, Karine Bastard, Lilian Beloti, Amina Frese, Johan P Turkenburg, et al.. A family of native amine dehydrogenases for the asymmetric reductive amination of ketones. Nature Catalysis, 2019, 2 (4), pp.324-333. ⟨10.1038/s41929-019-0249-z⟩. ⟨hal-02945525⟩
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