The study of vascular morphometry requires segmenting vessels with high precision. Of particular clinical interest is the morpho-metric analysis of arterial bifurcations in Adaptive Optics Ophthal-moscopy (AOO) images of eye fundus. In this paper, we extend our previous approach for segmenting retinal vessel branches to the segmentation of bifurcations. This enables us to recover the mi-crovascular tree and extract biomarkers that charactarize the blood flow. Segmentation results are shown to be within the range of intra-and inter-user variability, allowing a preliminary study on biomark-ers derived from vessel diameter estimates at arterial bifurcations.