Neurostimulation with nanosecond pulsed electric fields: A combined electrophysiology and multiphoton imaging approach in intact ganglia
Résumé
The effect of high power nanosecond pulsed electric fields (nsPEF) was examined on the complex neuronal assemblies of
intact leech ganglia. The medicinal leech midbody ganglion is a convenient experimental preparation that represents a real
neuronal network with neuronal diversity and glial cells. 10 ns pulses of different intensities ranging from 22.5 to 47 kV/cm
were applied to the ganglia body and alterations in natural electrical activity of Retzius cells were recorded with sharp glass
electrode in a current-clamp mode or multiphoton whole-ganglia imaging with calcium indicators. The lowest nsPEF
intensity tested resulted in stimulation of a single action potential (AP) whereas all higher intensities used led to bursts of
APs.