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Article Dans Une Revue International Immunology Année : 2019

Characterization of the developmental landscape of murine RORγt+ iNKT cells

Résumé

Invariant natural killer T (iNKT) cells expressing the retinoic acid receptor-related orphan receptor γt (RORγt) and producing IL-17 represent a minor subset of CD1d-restricted iNKT cells (iNKT17) in C57BL/6J (B6) mice. We aimed in this study to define the reasons for their low distribution and the sequence of events accompanying their normal thymic development. We found that RORγt+ iNKT cells have higher proliferation potential and a greater propensity to apoptosis than RORγt− iNKT cells. These cells do not likely reside in the thymus indicating that thymus emigration, and higher apoptosis potential, could contribute to RORγt+ iNKT cell reduced thymic distribution. Ontogeny studies suggest that mature HSAlow RORγt+ iNKT cells might develop through developmental stages defined by a differential expression of CCR6 and CD138 during which RORγt expression and IL-17 production capabilities are progressively acquired. Finally, we found that RORγt+ iNKT cells perceive a strong TCR signal that could contribute to their entry into a specific ‘Th17 like’ developmental program influencing their survival and migration. Overall, our study proposes a hypothetical thymic developmental sequence for iNKT17 cells, which could be of great use to study molecular mechanisms regulating this developmental program.

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Immunité innée
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hal-02401536 , version 1 (17-06-2020)

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Jihène Klibi, Shamin Li, Ludivine Amable, Claudine Joseph, Stéphane Brunet, et al.. Characterization of the developmental landscape of murine RORγt+ iNKT cells. International Immunology, 2019, 32 (2), pp.105-116. ⟨10.1093/intimm/dxz064⟩. ⟨hal-02401536⟩
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