Involvement of ceramide enriched domains in the targeted and non-targeted effects of HNSCC cancer cells
Résumé
Hypothesis: We investigated the relative contribution of targeted and non-targeted effects in the response of human head and neck cancer (HNSCC) cells to photon irradiation as well as the molecular mechanisms involved.
Methods: The SCC61 (radiosensitive) and SQ20B (radioresistant) cell lines were used. Targeted effects were measured in cells exposed to increased radiation doses (donor cells) while non-targeted effects were investigated in recipient cells. Recipient cells consisted of cells not exposed to X-rays, but grown in medium previously incubated for 2h with donor cells (conditioned medium). DNA double strand breaks (DNA DSBs) were measured in donor and recipient cells, using immunofluorescent detection of gamma-H2AX.