Systematic determination of transcription factor DNA-binding specificities in yeast - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2016

Systematic determination of transcription factor DNA-binding specificities in yeast

Résumé

Understanding how genes are regulated, decoding their "regulome", is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.
Fichier principal
Vignette du fichier
PDFsam_merge.pdf (4.28 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02356251 , version 1 (08-11-2019)

Identifiants

Citer

Lourdes Peña-Castillo, Gwenaël Badis. Systematic determination of transcription factor DNA-binding specificities in yeast. Frédéric Devaux. Yeast Functional Genomics, 1361, Humana Press, pp.203-225, 2016, Methods in Molecular Biology, 978-1-4939-3079-1. ⟨10.1007/978-1-4939-3079-1_12⟩. ⟨hal-02356251⟩
16 Consultations
45 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More