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Article Dans Une Revue Bioconjugate Chemistry Année : 2018

Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein

Résumé

Galectins (Gal) are a family of glycan-binding proteins characterized by their affinity for β-galactosides. Galectin-1 (Gal-1), a dimeric lectin with two galactoside-binding sites, regulates cancer progression and immune responses. Coordination chemistry has been engaged to develop versatile multivalent neoglycoconjugates for binding Gal-1. In this study we report a fast and original method to synthesize hybrid gold nanoparticles in which a hydrochloride lactose-modified chitosan, named CTL, is mixed with dicarboxylic acid-terminated polyethylene glycol (PEG), leading to shell-like hybrid polymer-sugar-metal nanoparticles (CTL-PEG-AuNPs). The aim of this paper is to preliminarily study the interaction of the CTL-PEG-AuNPs with a target protein, namely, Gal-1, under specific conditions. The molecular interaction has been measured by Transmission Electron Microscopy (TEM), UV–vis, and Raman Spectroscopy on a large range of Gal-1 concentrations (from 0 to 10–12 M). We observed that the interaction was strongly dependent on the Gal-1 concentration at the surface of the gold nanoparticles.

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Chimie Polymères
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Dates et versions

hal-02353585 , version 1 (07-11-2019)

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Qiqian Liu, Pasquale Sacco, Eleonora Marsich, Franco Furlani, Celia Arib, et al.. Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein. Bioconjugate Chemistry, 2018, 29 (10), pp.3352-3361. ⟨10.1021/acs.bioconjchem.8b00520⟩. ⟨hal-02353585⟩
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