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Article Dans Une Revue Toxicology and Applied Pharmacology Année : 2018

In vitro interaction and biocompatibility of titanate nanotubes with microglial cells

Résumé

Titanate nanotubes (TiONts) are promising agents for biomedical applications. Microglial activation and associated oxidative burst are major challenges in drug delivery applications across the brain. Here, TiONts were designed for drug delivery systems by functionalizing them with (3-aminopropyl) triethoxysilane (APTES), their interactions and biocompatibility were studied in vitro using murine microglial BV-2 cells. TiONts-APTES exposure resulted in increased ROS production and transient mitochondrial hyperpolarization. However, there was 2 no indication of microglial proliferation in BV-2 cells as suggested by cell cycle analysis and cell count. The internalization process of TiONts-APTES into cells by endocytosis vesicles and passive diffusion were proved by transmission electron microscopy (TEM) with and without amiloride, the endocytosis inhibiting agent. In addition, the TiONts-APTES exhibited good biocompatibility on microglial BV-2 cells as revealed by the morphology and viability analysis.
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Dates et versions

hal-02163529 , version 1 (09-03-2021)

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S. Sruthi, A. Loiseau, J. Boudon, F. Sallem, Lionel Maurizi, et al.. In vitro interaction and biocompatibility of titanate nanotubes with microglial cells. Toxicology and Applied Pharmacology, 2018, 353, pp.74-86. ⟨10.1016/j.taap.2018.06.013⟩. ⟨hal-02163529⟩
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