Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila - Archive ouverte HAL
Article Dans Une Revue Scientific Reports Année : 2015

Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila

Pierre-Alexandre Juan
  • Fonction : Auteur
  • PersonId : 782059
  • IdRef : 195154665
Laetitia Attaiech

Résumé

Natural transformation is the process by which bacteria can actively take up and integrate exogenous DNA thereby providing a source of genetic diversity. Under specific growth conditions the coordinated expression of several genes - a situation referred to as "competence" - allows bacteria to assemble a highly processive and dedicated system that can import high molecular weight DNA. Within the cell these large imported DNA molecules are protected from degradation and brought to the chromosome for recombination. Here, we report elevated expression of mreB during competence in the Gram-negative pathogen Legionella pneumophila. Interestingly a similar observation had previously been reported in the distantly-related Gram-positive organism Bacillus subtilis. MreB is often viewed as the bacterial actin homolog contributing to bacterial morphogenesis by coordinating peptidoglycan-synthesising complexes. In addition MreB is increasingly found to be involved in a growing number of processes including chromosome segregation and motor-driven motility. Using genetic and pharmacological approaches, we examined the possible role of MreB during natural transformation in L. pneumophila. Our data show that natural transformation does not require MreB dynamics and exclude a direct role of MreB filaments in the transport of foreign DNA and its recombination in the chromosome.
Fichier principal
Vignette du fichier
srep16033.pdf (2.47 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-02001317 , version 1 (15-10-2024)

Licence

Identifiants

Citer

Pierre-Alexandre Juan, Laetitia Attaiech, Xavier Charpentier. Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila. Scientific Reports, 2015, 5, pp.16033. ⟨10.1038/srep16033⟩. ⟨hal-02001317⟩
10 Consultations
0 Téléchargements

Altmetric

Partager

More