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Communication Dans Un Congrès Année : 2018

Effect of environmental ARG expression under selective pressure on ARG transfer to bacteria

Résumé

The continuous increase of antibiotic-resistant bacteria (ARBs) in medical clinics is a major global health concern. Although the use of antibiotics for human and animal treatment seems to be related to the spread of ARBs, antibiotic resistance dispersion is a natural environmental process. Furthermore, several studies have documented the influence of anthropogenic activity on the environmental “resistome”. Novel antibiotic resistance genes (ARGs) may emerge in the non-clinical environment and spread to the human microbiome, from where the probability of their transfer to human pathogens increases. Thus, we are interested in the environmental factors that influence the rate and probability of ARGs transfer from non-human microbiome bacteria to human microbiome bacteria in non-clinical environments. Here we report on the influence of in situ transcription rates on the transfer of genes in soil. In order to determine whether the transcription of ARGs under different selective pressures increases their transfer rate to human microbiome bacteria, natural soils and soils incubated with antibiotics were inoculated with a labelled strain of E. coli. At different time points, these E. coli were isolated from soil microcosms by flow cytometry and a combination of metatranscriptomics and qPCR was used to evaluate the transfer of environmental ARG to E. coli as a function of their transcription rate in the donor bacteria. This approach provided a model for the study of environmental ARG transfer to human microbiome bacteria directly in soil and highlights the importance of gene expression monitoring in risk assessment studies.
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Dates et versions

hal-01973591 , version 1 (08-01-2019)

Identifiants

  • HAL Id : hal-01973591 , version 1

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Concepcion Sanchez-Cid, Laure Franqueville, Timothy M. Vogel. Effect of environmental ARG expression under selective pressure on ARG transfer to bacteria. ISME17, Aug 2018, Leipzig, Germany. pp.23. ⟨hal-01973591⟩
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