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Journal Articles Applied Biochemistry and Biotechnology Year : 2014

Electroanalysis may be used in the Vanillin Biotechnological Production

Abstract

This study shows that electroanalysis may be used in vanillin biotechnological production. As a matter of fact, vanillin and some molecules implicated in the process like eugenol, ferulic acid, and vanillic acid may be oxidized on electrodes made of different materials (gold, platinum, glassy carbon). By a judicious choice of the electrochemical method and the experimental conditions the current intensity is directly proportional to the molecule concentrations in a range suitable for the biotechnological process. So, it is possible to imagine some analytical strategies to control some steps in the vanillin biotechnological production: by sampling in the batch reactor during the process, it is possible to determine out of line the concentration of vanillin, eugenol, ferulic acid, and vanillic acid with a gold rotating disk electrode, and low concentration of vanillin with addition of hydrazine at an amalgamated electrode. Two other possibilities consist in the introduction of electrodes directly in the batch during the process; the first one with a gold rotating disk electrode using linear sweep voltammetry and the second one requires three gold rotating disk electrodes held at different potentials for chronoamperometry. The last proposal is the use of ultramicroelectrodes in the case when stirring is not possible.
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Dates and versions

hal-01887688 , version 1 (04-10-2018)

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William Giraud, Marie Mirabel, Maurice Comtat. Electroanalysis may be used in the Vanillin Biotechnological Production. Applied Biochemistry and Biotechnology, 2014, 172 (4), pp.1953-1963. ⟨10.1007/s12010-013-0631-2⟩. ⟨hal-01887688⟩
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