A new bone tissue process using supercritical carbon dioxide extraction has been checked for viral inactivation and allografts, thus processed, for bone integration. Four viruses, human immunodeficiency virus type I (HIV-I), Sindbis virus, Polio Sabin type-I virus, and Pseudorabies virus (PRV) and four processing stages, supercritical CO2, hydrogen peroxide, sodium hydroxide and ethanol treatments were tested. The cumulated reduction factors (log10) were the following: >18.2 for Sindbis virus, >24.4 for polio virus, >17.6 for PRV and >14.2 for HIV-I. Such processed allografts were implanted into sheep leading to a much faster osseointegration in comparison with non-treated allografts. Better graft incorporation in addition to viral safety suggest that this process could become a new way for processing bank bones, alternatively or additionally, to the procedures presently used.