Co-liposomes comprising a lipidated multivalent RGD-peptide and a cationic gemini cholesterol induce selective gene transfection in αvβ3 and αvβ5 integrin receptor-rich cancer cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of materials chemistry‎ B Année : 2014

Co-liposomes comprising a lipidated multivalent RGD-peptide and a cationic gemini cholesterol induce selective gene transfection in αvβ3 and αvβ5 integrin receptor-rich cancer cells

Résumé

The αvβ3 and αvβ5 integrins, transmembrane glycoprotein receptors, are over-expressed in numerous tumors and in endothelial cells that constitute tumor blood vessels. As this protein selectively binds to the Arg–Gly–Asp (RGD) sequence containing peptides, it is an attractive way to target tumors. Herein we have developed novel formulations for integrin mediated selective gene delivery. These formulations are composed of a novel palmitoylated tetrameric RGD containing scaffold (named RAFT-RGD), cationic gemini cholesterol (GL5) and a natural helper lipid 1,2-dioleoyl-L-α-glycero-3-phosphatidylethanolamine (DOPE). We have optimized a co-liposomal formulation to introduce the multivalent RGD-containing macromolecule in GL5: DOPE (GL5D) mixture to produce GL5D–RGD. We have unambiguously shown the selectivity of these formulations towards cancer cells that over express αvβ3 and αvβ5 integrins. Two reporter plasmids, pEGFP-C3 and PGL-3, were employed for the transfection experiments and it was shown that GL5D–RGD liposomes increased exclusively the transfection in αvβ3 and αvβ5-overexpressing HeLa cells.
Fichier principal
Vignette du fichier
C4TB00701H.pdf (1.14 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01652173 , version 1 (28-03-2022)

Identifiants

Citer

Santosh Misra, Paturu Kondaiah, Santanu Bhattacharya, Didier Boturyn, Pascal Dumy. Co-liposomes comprising a lipidated multivalent RGD-peptide and a cationic gemini cholesterol induce selective gene transfection in αvβ3 and αvβ5 integrin receptor-rich cancer cells. Journal of materials chemistry‎ B, 2014, 2 (35), pp.5758 - 5767. ⟨10.1039/C4TB00701H⟩. ⟨hal-01652173⟩
66 Consultations
34 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More