Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [ 11 C]-( R )-PK11195 PET and MRI
Résumé
Inflammation may play a role in the development of epilepsy after brain insults. [ 11 C]-(R)-PK11195 binds to TSPO, expressed by activated microglia. We quantified [ 11 C]-(R)-PK11195 binding during epileptogenesis after pilocarpine-induced status epilepticus (SE), a model of temporal lobe epilepsy. Nine male rats were studied thrice (D0-1, D0 þ 6, D0 þ 35, D0 ¼ SE induction). In the same session, 7T T2-weighted images and DTI for mean diffusivity (MD) and fractional anisotropy (FA) maps were acquired, followed by dynamic PET/ CT. On D0 þ 35, femoral arterial blood was sampled for rat-specific metabolite-corrected arterial plasma input functions (AIFs). In multiple MR-derived ROIs, we assessed four kinetic models (two with AIFs; two using a reference region), standard uptake values (SUVs), and a model with a mean AIF. All models showed large (up to twofold) and significant TSPO binding increases in regions expected to be affected, and comparatively little change in the brainstem, at D0 þ 6. Some individuals showed increases at D0 þ 35. AIF models yielded more consistent increases at D0 þ 6. FA values were decreased at D0 þ 6 and had recovered by D0 þ 35. MD was increased at D0 þ 6 and more so at D0 þ 35. [ 11 C]-(R)-PK11195 PET binding and MR biomarker changes could be detected with only nine rats, highlighting the potential of longitudinal imaging studies.
Domaines
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