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Communication Dans Un Congrès Année : 2016

Joint 3D alignment-reconstruction multi-scale approach for cryo electron tomography

Résumé

3D volume reconstruction in cryo-electron tomography is possible by using Transmission Electron Microscope (TEM) images from different tilt angles. The misalignment of these images is one of the limits to the quality of the reconstructed object. There are many alignment techniques to deal with this problem. Their common feature is to correct the 2D geometric transformation in the projection images. Nevertheless , 3D geometric transformation can occur in the TEM acquisition including tilt angular uncertainty. In this paper, we proposed a new multi-scale approach based on a Conjugate Gradient optimization of a cost function between the 3D reconstructed and the projection images with the purpose to find all the 3D parameters of geometric transformation. Tests on synthetic and real data prove the accuracy of our geometric transformation estimation.
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Dates et versions

hal-01318729 , version 1 (19-05-2016)

Identifiants

  • HAL Id : hal-01318729 , version 1

Citer

Hmida Rojbani, Etienne Baudrier, Benoît Naegel, Loïc Mazo, Atef Hamouda. Joint 3D alignment-reconstruction multi-scale approach for cryo electron tomography. IEEE International Symposium on Biomedical Imaging (ISBI 2016), Apr 2016, Prague, Czech Republic. ⟨hal-01318729⟩
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