This paper aims to reconstruct the vegetation history of a middle mountain – the Morvan (Burgundy, France) – since the Neolithic (i.e. 7000 years). The results of palynological and non-pollen palynomorphs (NPPs) analysis performed on 10 peat cores document the main phases of human agro-pastoral practices and natural resources management which drove the formation of the cultural landscape of the Morvan Massif over time. To document the history of human activities at a regional scale, particular attention was given to the determination, the characterization and the graphical depiction of phrases of human impact. Both the quantification of the impact of human pressure and the chronological uncertainty of the different phases identified by pollen and NPP analysis were taken into account. This study reveals a clear human presence during periods that were formerly poorly documented, such as the onset of the Neolithic period, the Bronze Age, the early Iron Age and the early Middle Ages. It also sheds new light over periods for which there is better archaeological and historical knowledge, such as the widespread occupation of the massif in the late Iron Age, the cultivation of chestnut from the 11th to the 16th century and the impact of modern firewood exploitation on the forest.