Analysis of the spatial organization of molecules with robust statistics - Archive ouverte HAL
Article Dans Une Revue PLoS ONE Année : 2013

Analysis of the spatial organization of molecules with robust statistics

Résumé

One major question in molecular biology is whether the spatial distribution of observed molecules is random or organized in clusters. Indeed, this analysis gives information about molecules' interactions and physical interplay with their environment. The standard tool for analyzing molecules' distribution statistically is the Ripley's K function, which tests spatial randomness through the computation of its critical quantiles. However, quantiles' computation is very cumbersome, hindering its use. Here, we present an analytical expression of these quantiles, leading to a fast and robust statistical test, and we derive the characteristic clusters' size from the maxima of the Ripley's K function. Subsequently, we analyze the spatial organization of endocytic spots at the cell membrane and we report that clathrin spots are randomly distributed while clathrin-independent spots are organized in clusters with a radius of 2 mm, which suggests distinct physical mechanisms and cellular functions for each pathway.
Fichier principal
Vignette du fichier
journalpone0080914.pdf (699.85 Ko) Télécharger le fichier
Origine Publication financée par une institution
Loading...

Dates et versions

hal-01197614 , version 1 (30-11-2016)

Licence

Identifiants

Citer

Thibault Lagache, Gabriel Lang, Nathalie Sauvonnet, Jean-Christophe Olivo-Marin. Analysis of the spatial organization of molecules with robust statistics. PLoS ONE, 2013, 8 (12), 7 p. ⟨10.1371/journal.pone.0080914⟩. ⟨hal-01197614⟩
254 Consultations
112 Téléchargements

Altmetric

Partager

More