H3S10 phosphorylation marks constitutive heterochromatin during interphase in early mouse embryos until the 4-cell stage - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Reproduction and Development Année : 2012

H3S10 phosphorylation marks constitutive heterochromatin during interphase in early mouse embryos until the 4-cell stage

Résumé

Phosphorylation of histone H3 at Ser10 (H3S10P) has been linked to a variety of cellular processes, such as chromosome condensation and gene activation/silencing. Remarkably, in mammalian somatic cells, H3S10P initiates in the pericentromeric heterochromatin during the late G2 phase, and phosphorylation spreads throughout the chromosomes arms in prophase, being maintained until the onset of anaphase when it gets dephosphorylated. Considerable studies have been carried out about H3S10P in different organisms; however, there is little information about this histone modification in mammalian embryos. We hypothesized that this epigenetic modification could also be a marker of pericentromeric heterochromatin in preimplantation embryos. We therefore followed the H3S10P distribution pattern in the G1/S and G2 phases through the entire preimplantation development in in vivo mouse embryos. We paid special attention to its localization relative to another pericentromeric heterochromatin marker, HP1β and performed immunoFISH using specific pericentromeric heterochromatin probes. Our results indicate that H3S10P presents a remarkable distribution pattern in preimplantation mouse embryos until the 4-cell stage and is a better marker of pericentromeric heterochromatin than HP1β. After the 8-cell stage, H3S10P kinetic is more similar to the somatic one, initiating during G2 in chromocenters and disappearing upon telophase. Based on these findings, we believe that H3S10P is a good marker of pericentromeric heterochromatin, especially in the late 1- and 2-cell stages as it labels both parental genomes and that it can be used to further investigate epigenetic regulation and heterochromatin mechanisms in early preimplantation embryos.
Fichier principal
Vignette du fichier
2012_Ribeiro-Masson_Journal of Reproduction and Development_1 (1.87 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01190305 , version 1 (28-05-2020)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

  • HAL Id : hal-01190305 , version 1
  • PRODINRA : 190885
  • PUBMED : 22572731
  • WOS : 000308055000013

Citer

Karlla Mason, Claire Boulesteix, Renaud Fleurot, Tiphaine Aguirre-Lavin, Pierre Adenot, et al.. H3S10 phosphorylation marks constitutive heterochromatin during interphase in early mouse embryos until the 4-cell stage: H3S10P marks constitutive heterochromatin in early mouse embryos. Journal of Reproduction and Development, 2012, 58 (4), pp.467-75. ⟨hal-01190305⟩
55 Consultations
58 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More